Laser-Assisted Bioprinting (LAB)

  High-speed photography of a LIFT process over a distance of 1100 µm. A laser pulse induces a vapour bubble in the transfer substrate (2 µs), which collapses shortly afterwards (7 µs) and forms a jet (20 µs). Copyright: © Chair for Lasertechnology LLT, RWTH Aachen University. High-speed photography of a LIFT process over a distance of 1100 µm. A laser pulse induces a vapour bubble in the transfer substrate (2 µs), which collapses shortly afterwards (7 µs) and forms a jet (20 µs).

Laser-Assisted Bioprinting (LAB)

Laser-Induced Forward Transfer (LIFT) is a laser-based process for the contactless transfer of material. Due to its high precision and low requirements regarding the rheological properties of the inks used, LIFT offers the best prerequisites for a wide range of applications. In laser-assisted bioprinting (LAB), LIFT of liquids is used for microdosing as well as for the isolation and positioning of single cells, cell clusters and spheroids.

At the Chair of Laser Technology, the fundamentals of laser-assisted bioprinting are being investigated in more detail. Special attention is paid to LIFT with adapted laser wavelengths to avoid the need for an additional absorber. By combining basic research with direct process observation using high-speed photography and microscopy, new fields of application for LIFT of biological materials will be identified and existing applications will be developed further. In close cooperation with the Fraunhofer ILT, these technologies can be investigated and advanced from the idea to market maturity.

Research Focus

  • Analysis of the gel- and laser-parameters in the process.

  • Controlled fluid transfer in the picoliter and attoliter range.
  • Investigation of the transfer of gels and fluids into and out of microstructures.
  • Perspective: culturing of transferred single cells and spheroids for 4D bioprinting of test systems and tissue-like structures
  Glass transfer carrier in a LIFT cell sorting system above a 96-well microtitre plate. Copyright: © Fraunhofer ILT, Aachen. Glass transfer carrier in a LIFT cell sorting system above a 96-well microtitre plate.

Publications

  • Jaeckle, E., Brauchle, E., Nottrodt, N., Wehner, M., Lensing, R., Gillner, A., ... & Burger-Kentischer, A. (2020). Towards automation in biologics production via Raman micro-spectroscopy, laser-induced forward cell transfer and surface-enhanced Raman spectroscopy. Journal of Biotechnology, 323, 313-321. DOI: 10.1016/j.jbiotec.2020.09.001.

  • Riester, D., Budde, J., Gach, C., Gillner, A., & Wehner, M. (2016). High speed photography of laser induced forward transfer (LIFT) of single and double-layered transfer layers for single cell transfer. Journal of Laser Micro Nanoengineering, 11(2), 199. DOI: 10.2961/jlmn.2016.02.0010.

Contact

Name

Carina Schemmer

Phone

work
0241 80 40428

Email

E-Mail